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A new rapid method for isolation of naturally occurring porphyrins and their quantitation after high performance liquid chromatography

✍ Scribed by Z.J. Petryka; C.J. Watson

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
312 KB
Volume
84
Category
Article
ISSN
0003-2697

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✦ Synopsis

High performance liquid chromatography (HPLC) has been used to separate porphyrin methyl esters isolated from porphyric patients. Fecal porphyrins were esterified directly with boron trifluoride; urinary porphyrins were absorbed on talcum and then treated with boron trifluoride. HPLC permits rapid, efficient, and quantitative detection and identification of porphyrins in complex natural mixtures.

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