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A simple and rapid method of quantitative analysis of phosphoamino acids by high-performance liquid chromatography

✍ Scribed by Nick Morrice; Alastair Aitken

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
412 KB
Volume
148
Category
Article
ISSN
0003-2697

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✦ Synopsis

A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue of this uv-active eluant. The technique is sensitive to 200 pmol of phosphoamino acid and has been shown to be directly applicable to the analysis of isolated phosphoproteins. 0 1985 Academic press, 1nc.

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