Laser-induced tobacco protoplast fusion
β Scribed by Yinmei Li; Lijie Guan; Liren Lou; Guoqiang Cui; Yuan Yao; Haowei Wang; Chuanshun Cao; Runlong Lu; Xi Chen
- Book ID
- 105640274
- Publisher
- Science in China Press (SCP)
- Year
- 1999
- Tongue
- English
- Weight
- 439 KB
- Volume
- 42
- Category
- Article
- ISSN
- 1674-7305
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Direct selection for recombinants by supplemented minimal media from polyethylene-glycol (PEG)-induced fusion of protoplasts of polyauxotrophic strains of B. megaterium revealed striking physiological influences on the yield of recombinants. Cytoplasmic state of the protoplasts to be fused, rather t
Intergeneric hybridization between Pleurotus ostreatus and Schizophyllum commune was studied using PEG-induced fusion. The fusion of protoplasts from auxotrophic mutant strains resulted in the formation of fusion hybrids in the frequencies of 3.6 to 7.3Γ10(-5). Most of these fusion hybrids were mono