Intergeneric hybridization betweenPleurotus ostreatusandSchizophyllum communeby PEG-induced protoplast fusion

✍ Scribed by J. Zhao; S. T. Chang

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 560 KB
• Volume: 12
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00327717

✦ Synopsis

Intergeneric hybridization between Pleurotus ostreatus and Schizophyllum commune was studied using PEG-induced fusion. The fusion of protoplasts from auxotrophic mutant strains resulted in the formation of fusion hybrids in the frequencies of 3.6 to 7.3×10(-5). Most of these fusion hybrids were monokaryotic and sterile and no heterokaryosis occurred. Most fusants showed a significantly higher nuclear DNA content when compared to parental strains and no diploids (parent 1 genome plus parent 2 genome) were found. Some fusion hybrids revealed both parental fragments in nuclear and mitochondrial rDNA PCR profiles. AP-PCR (Arbitrarily-primed Polymerase Chain Reaction) fingerprints also indicated that most of the fusion products were recombinant hybrids.