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Large-scale transient gene expression in mammalian cells: Cost-effective preparation of plasmid DNA

✍ Scribed by Weiwen Luo; Hui Ching Hia; Xiangming Sun

Book ID
118473134
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
52 KB
Volume
136
Category
Article
ISSN
0168-1656

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p