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Large scale gene expression profiling of metabolic shift of mammalian cells in culture

✍ Scribed by Rashmi Korke; Marcela de Leon Gatti; Ally Lei Yin Lau; Justin Wee Eng Lim; Teck Keong Seow; Maxey Ching Ming Chung; Wei-Shou Hu

Book ID
118470973
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
263 KB
Volume
107
Category
Article
ISSN
0168-1656

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p