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Transient Gene Expression in Suspension HEK-293 Cells: Application to Large-Scale Protein Production

โœ Scribed by Lucia Baldi; Natalie Muller; Sylviane Picasso; Raymond Jacquet; Philippe Girard; Huy Phan Thanh; Elisabeth Derow; Florian M. Wurm


Book ID
109387141
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
125 KB
Volume
21
Category
Article
ISSN
8756-7938

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We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us