Enhancement of transient gene expression by fed-batch culture of HEK 293 EBNA1 cells in suspension
β Scribed by Xiangming Sun; Peen Ern Goh; Kathy T. K. Wong; Tetsushi Mori; Miranda G. S. Yap
- Book ID
- 106363525
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 197 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0141-5492
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We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us
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