Large-scale preparation and characterization of zinc-substituted hydroxyapatite for metal affinity chromatography
β Scribed by Yukiko Murakami; Ken Sugo; Tomohiko Yoshitake; Masahiro Hirano; Tsuneo Okuyama
- Book ID
- 119367180
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 935 KB
- Volume
- 103
- Category
- Article
- ISSN
- 1383-5866
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the inβdepth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig
The reaction parameters for preparation of Cibacron Blue F3G-A polyethylene glycol have been studied, including temperature, concentration of reactants, and addition of neutral salt (Na,SO,) as well as ethanol. The yield of dyepolymer is strongly dependent on temperature and time of reaction. Prepar