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Large-scale Analysis of in Vivo Phosphorylated Membrane Proteins by Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry

โœ Scribed by Nuhse, T. S.


Book ID
125535359
Publisher
The American Society for Biochemistry and Molecular Biology
Year
2003
Tongue
English
Weight
713 KB
Volume
2
Category
Article
ISSN
1535-9476

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Consecutive enzymatic reactions on analytes affinity-bound to immobilized metal ion beads with subsequent direct analysis of the products by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry have been used for detecting protein synthesis errors occuring at the N-terminus.