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Identification of protein phosphorylation sites by combination of elastase digestion, immobilized metal affinity chromatography, and quadrupole-time of flight tandem mass spectrometry

✍ Scribed by Andreas Schlosser; Jochen Bodem; Dirk Bossemeyer; Ingrid Grummt; Wolf Dieter Lehmann


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
116 KB
Volume
2
Category
Article
ISSN
1615-9853

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## On -line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization-mass spectrometry (IMAC/CE/ESI-MS) offers selective preconcentration of phosphorylated peptides with identification of the phosphorylated amino acid(s). The preconcentration provides low concent