๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Large-scale insect cell culture: methods, applications and products

โœ Scribed by Mattheus F.A. Goosen


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
435 KB
Volume
2
Category
Article
ISSN
0958-1669

No coin nor oath required. For personal study only.

โœฆ Synopsis


The primary development in large-scale insect cell culture over the past year has been the continuing accumulation of documented evidence (fundamental and applied) that conventional aerated stirred-tank and air-lift bioreactors may be employed for insect cell cultivation and recombinant protein production, provided that air sparging, agitation, and the addition to the medium of Pluronic F-68 and methyl cellulose polymers are carefully controlled.


๐Ÿ“œ SIMILAR VOLUMES


Large-scale mammalian cell culture: meth
โœ Raymond E. Spier ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 509 KB

Animal cell cultures are used to generate products of enormous biotechnological value. These systems rely on conventional manufacturing techniques using organisms that are the result of either cell fusions or genetic engineering. A wealth of new techniques has allowed improvements and developments t

Large-scale insect cell culture
โœ Mattheus F.A. Goosen ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 605 KB

Significant advances have been made over the past year in our understanding of the protective mechanisms, both fluid-mechanical and biological, of media additives on suspended animal cells. The degree of protection offered by different additives, such as pluronic polyol, appears to be cell-type depe

Industrial choices for protein productio
โœ Lily Chu; David K Robinson ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 67 KB

Traditional barriers to large-scale mammalian culture have now been addressed, with the standard stirred-tank reactor emerging as industry's technology of choice. The issues of adapting cells to suspension culture, shear sensitivity and oxygen supply have been largely resolved. But for many low-volu

Large scale fermentation and alkaloid pr
โœ Otmar Schiel; Jochen Berlin ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 470 KB

Cell cultures of Catharanthus roseus were scaled up to volumes of 50001 using conventional reactors equipped with fiat-blade impellers. The behavior of the fermenter grown cells was compared with corresponding shake flask experiments with respect to growth and indole alkaloid inducibility and produc