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Large scale fermentation and alkaloid production of cell suspension cultures ofCatharanthus roseus

✍ Scribed by Otmar Schiel; Jochen Berlin

Publisher: Springer
Year: 1987
Tongue: English
Weight: 470 KB
Volume: 8
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00043152

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✦ Synopsis

Cell cultures of Catharanthus roseus were scaled up to volumes of 50001 using conventional reactors equipped with fiat-blade impellers. The behavior of the fermenter grown cells was compared with corresponding shake flask experiments with respect to growth and indole alkaloid inducibility and production. The limits and problems of transferring shake flask experiments of culture systems such as Catharanthus, in which alkaloid production depends greatly upon the physiological state of the cells, to large scale multistage processes is discussed.

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