Indole alkaloid production by hairy root cultures ofCatharanthus roseus: Growth kinetics and fermentation
β Scribed by L. Toivonen; M. Ojala; V. Kauppinen
- Publisher
- Springer Netherlands
- Year
- 1990
- Tongue
- English
- Weight
- 357 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-5492
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Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by
## Abstract Nitric oxide (NO) is known as a signaling molecule involved in elicitorβinduced defense responses of plants. Sodium nitroprusside (SNP), a donor of NO, stimulates catharanthine formation in Catharanthus roseus cells.1 Two important terpenoid indole alkaloids produced in small quantities
Cell cultures of Catharanthus roseus were scaled up to volumes of 50001 using conventional reactors equipped with fiat-blade impellers. The behavior of the fermenter grown cells was compared with corresponding shake flask experiments with respect to growth and indole alkaloid inducibility and produc
The relationship between the morphology and indole alkaloid production of Catharanthus roseus cells was investigated. Eleven cell lines were randomly selected from protoplast-derived clones. In each line, most of the cells maintained only one of the two shapes, either spherical or cylindrical. The c