Large-scale mammalian cell culture: methods, applications and products
β Scribed by Raymond E. Spier
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 509 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
β¦ Synopsis
Animal cell cultures are used to generate products of enormous biotechnological value. These systems rely on conventional manufacturing techniques using organisms that are the result of either cell fusions or genetic engineering. A wealth of new techniques has allowed improvements and developments to be made in culture medium composition, cell modification, and bioreactor design and operation. This progress is expected to be commercially exploited as new products reach the market place.
π SIMILAR VOLUMES
The primary development in large-scale insect cell culture over the past year has been the continuing accumulation of documented evidence (fundamental and applied) that conventional aerated stirred-tank and air-lift bioreactors may be employed for insect cell cultivation and recombinant protein prod
## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100βL scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p
## Abstract A flatβbed hollowβfiber cell culture system has been developed which maximizes the utilization of the large fiber surface while diminishing significantly the problems inherent in a cartridgeβtype reactor. The reactor core consists of a shallow bed of hollow fibers sandwiched between two