Restriction fragment length polymorphism (RFLP) of Ha-
In the present studies, the role of Ha-ras RFLP in cancer ras gene was surveyed by Southern blot analysis in leukocyte susceptibility and expression of Ha-rus gene was investigated DNA from 55 normal individuals and 58 urothelial cancer in urothelial cancer in japan, patients in Japan. Three common alleles and 4 rare alleles were classified. The frequency of common alleles in normal Japanese individuals differed from that in Caucasians previously reported; a 7.2-7.5-kb BamHl fragment of common allele was not observed in Japanese individuals. No significant increase in frequency of the rare Ha-ras allele was observed in the group of cancer patients. Moreover, no significant difference in frequency was observed for the 3 COrnmOn alleles.
Tumor DNA was ras locus, decreased intensity of one band, indicating partial loss of one allele in tumor DNA, was observed. In tumors with either deletion of one Ha-ras allele o r a rare Ha-ras carcinomas of the bladder and 3 adenocarcinomas of the bladallele, expression of Ha-ras gene was examined by Northern der. In 30 urothelial cancer patients (25 with bladder cancer, blot analysis. Such genetic alterations did not always result in 3 with ureter cancer and 2 with renal pelvic cancer), tumor a marked increase in Ha-ras expression. These data suggest DNA, as well as DNA from peripheral blood leukocytes, was that these genetic alterations are not directly related to Ha-also subjected to analysis.
ras expression, and that RFLP of Ha-ras gene is not a useful genetic marker for urothelial cancer. On the other hand, Tumor tissues and cell line deletion of one Ha-ras allele was observed in I of 5 cases of T~~~~~ were obtained by surgical resection and stored at bladder cancer and in 2 of 3 cases of renal pelvic cancer, -80°C until DNA and RNA extraction, The human bladder carcinoma cell line T24 (Bubenik et al., 1973) was obtained suggesting that that deletion may be important in the development of urothelial cancer.
On the Other hand, loss Of One Ha-ras >To whom reprint requests should be sent, at the Dept. of Pathology,