Labeling of small molecules with fluorescein
โ Scribed by Ian Simpson
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 126 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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We determined the conditions for labeling rabbit Fab' with fluorescein isothiocyanate to provide maximal fluorescence intensity compatible with the preservation of integrity of its single thiol residue. We found that we could reproducibly substitute Fab' with two fluorescein moieties in 30 min at ro
Fluorescein labeling of peptides and proteins is required for numerous biophysical or biological experiments such as fluorescence microscopy, fluorescence resonance energy transfer (FRET) or fluorescence imaging. The commonly used strategy relied on the coupling of the dye reagent followed by a gel