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One-pot labeling and purification of peptides and proteins with fluorescein maleimide

✍ Scribed by Eric Vivès; Bernard Lebleu

Publisher
Elsevier Science
Year
2003
Tongue
French
Weight
107 KB
Volume
44
Category
Article
ISSN
0040-4039

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✦ Synopsis

Fluorescein labeling of peptides and proteins is required for numerous biophysical or biological experiments such as fluorescence microscopy, fluorescence resonance energy transfer (FRET) or fluorescence imaging. The commonly used strategy relied on the coupling of the dye reagent followed by a gel filtration to recover the labeled molecule. Here we report a simplified method for the labeling of peptides and proteins on a cysteine residue and their purification. The method is based on the precipitation of peptides and proteins in acetone, fluorescein maleimide being soluble in this solvent. The excess of dye is fully eliminated after a couple of acetone washes and the precipitated peptide or protein is readily recovered.

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