## Abstract Actin and tropomyosin, purified from both muscle and brain, and Ξ±βactinin, purified from muscle, have been labeled in vitro by reductive methylation to specific activities of greater than 10^5^ dpm/ΞΌg protein. Actin so modified bound DNase I and polymerized identically to unmodified act
Labeling of pigeon erythrocyte membrane proteins by low-level reductive methylation
β Scribed by Steven W. Jones; George A. Vidaver
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 731 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Thaumatin I has been labelled by reductive methylation of four of the E-amino groups of the lysine residues in the protein with tritiated sodium borohydride.Methy1ation with unlabelled sodium borohydride showed that up to seven amino groups can be methylated without loss of sweetness intensity. The
have demonstrated that reductive methylation with labeled formaldehyde and NaCNBH, provides a simple method for specifically labeling the amino groups of proteins using extremely mild reaction conditions. However, cyanide, which is one of the products of the reaction, reduces labeling efficiency by