✦ LIBER ✦
Tritium labelling of Thaumatin I, a sweet-tasting protein from Thaumatococcus daniellii benth, by reductive methylation
✍ Scribed by H. van Der Wel; A. Wiersma; J. N. Brouwer
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- French
- Weight
- 187 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Thaumatin I has been labelled by reductive methylation of four of the E-amino groups of the lysine residues in the protein with tritiated sodium borohydride.Methy1ation with unlabelled sodium borohydride showed that up to seven amino groups can be methylated without loss of sweetness intensity. The yield of the tritium-labelled methylated Thaumatin I was 31% with a tritium incorporation of 4.7%. Its specific radioactivity was 12.8 Ci/mmol.