l-Glutamine transport in native vesicles isolated from Ehrlich ascites tumor cell membranes
✍ Scribed by Miguel A. Medina; Ana R. Quesada; Ignacio Núñez Castro
- Publisher
- Springer US
- Year
- 1991
- Tongue
- English
- Weight
- 430 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0145-479X
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton X‐100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium
## Abstract To obtain a clearer concept of the mechanism of organic solute transport in mammalian cells, we have attempted to reconstitute a functional transport system for amino acids from plasma membranes of Ehrlich ascites cells. Purified plasma membranes were dissolved in 2% Na cholate—4 M urea
Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and aff
Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o