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Na+-Dependent amino acid transport in plasma membrane vesicles from ehrlich ascites cells

✍ Scribed by Marco Colombini; Rose M. Johnstone

Publisher
Springer
Year
1974
Tongue
English
Weight
899 KB
Volume
15
Category
Article
ISSN
0022-2631

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πŸ“œ SIMILAR VOLUMES

Uptake of amino acids in reconstituted v
Uptake of amino acids in reconstituted vesicles derived from plasma membranes of ehrlich ascites cells
✍ R. M. Johnstone; Claudette Bardin πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 357 KB

## Abstract To obtain a clearer concept of the mechanism of organic solute transport in mammalian cells, we have attempted to reconstitute a functional transport system for amino acids from plasma membranes of Ehrlich ascites cells. Purified plasma membranes were dissolved in 2% Na cholateβ€”4 M urea

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## Abstract Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton X‐100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium

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## Abstract Vincristine, other periwinkle alkaloids, and colchicine partially inhibit the energy dependent transport of α‐aminoisobutyric acid in Ehrlich ascites tumor cells. The properties of this phenomenon were characterized in detail for vincristine. Maximum depression of the steady‐state intra