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Kinetics of Mu DNA synthesis

✍ Scribed by Wijffelman, Carel ;Lotterman, Bep

Book ID: 104729486
Publisher: Springer
Year: 1977
Tongue: English
Weight: 481 KB
Volume: 151
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00338691

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✦ Synopsis

Mu specific DNA synthesis starts at 10 min after infection. All essentail amber mutants of Mu were tested for the ability to replicate in a non permissive host. Except for the amber mutants A and B, which were already known to be blocked in Mu DNA synthesis (Wijffelman et al., 1974), all the other mutants showed normal Mu DNA replication. Using mitomycin C-treated cells Mu DNA synthesis was found to start at about 20 min after induction. However using the much more sensitive method of DNA-RNA hybridization, it was found that the DNA synthesis starts already at 10 min after induction, and that at 20 min after induction about 7 copies of the Mu DNA are present per cell.

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