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Kinetic characterization of branched chain ketoacid dehydrogenase

โœ Scribed by Bert Boyer; Richard Odessey


Book ID
115708853
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
743 KB
Volume
285
Category
Article
ISSN
0003-9861

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Purification of branched chain ฮฑ-ketoaci
โœ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s