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Killer DNA plasmids of the yeastKluyveromyces lactis

✍ Scribed by Micheline Wésolowski; Angela Algeri; Hiroshi Fukuhara

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
319 KB
Volume
5
Category
Article
ISSN
0172-8083

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✦ Synopsis

The killer character of Kluyverornyces lactis is cytoplasmically inherited. Two DNA plasmids kl and k2 (8.8 and 13.4 kilobase pairs, respectively) are associated with the killer system. Either cytoplasmic or nuclear mutations can lead to a loss of the killer activity. Two cytoplasmic non-killer mutants have been specifically studied. When they were crossed to each other, the killer phenotype was restored. Genetic analysis led us to conclude that each of the two mutants carried a mutation in the kl plasmid and that the recovery of killer phenotype was due to genetic recombination of the plasmids, and not to complementation.

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