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Structure of a linear plasmid of the yeastKluyveromyces lactis; Compact organization of the killer genome

โœ Scribed by Frederic Sor; Hiroshi Fukuhara


Publisher
Springer-Verlag
Year
1985
Tongue
English
Weight
870 KB
Volume
9
Category
Article
ISSN
0172-8083

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The killer character of Kluyveromyces lactis is associated with a cytoplasmic genetic element which confers on the host cells: a) the capacity to kill sensitive cells of various yeast by producing a diffusible toxin, and b) the immunity to that toxin. The killing activity is associated with the pres

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We have studied the structure of the two linear DNA plasmids, kl and k2, present in killer strains of Kluyveromyces lactis. Two killer strains of different origins, CBS 2359 and IFO 1267 were examined. For both strains, identical restriction maps of kl and k2 DNA were obtained. Several restriction s

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The killer character of Kluyverornyces lactis is cytoplasmically inherited. Two DNA plasmids kl and k2 (8.8 and 13.4 kilobase pairs, respectively) are associated with the killer system. Either cytoplasmic or nuclear mutations can lead to a loss of the killer activity. Two cytoplasmic non-killer muta

Palindrome-hairpin linear plasmids posse
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The yeast Kluyveromyces lactis harboring linear DNA plasmids pGKL1 and pGKL2 exhibits killer and killer-resistant phenotypes. Two new linear plasmids pK192L and pK192S were found in the weak killer mutant KUV192 induced by UV irradiation. pK192S was always accompanied by pK192L in subclones of KUV19