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Electroporation of the yeastKluyveromyces lactis

✍ Scribed by P. L. Bolen; J. E. McCutchan


Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
264 KB
Volume
6
Category
Article
ISSN
0951-208X

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We have studied the structure of the two linear DNA plasmids, kl and k2, present in killer strains of Kluyveromyces lactis. Two killer strains of different origins, CBS 2359 and IFO 1267 were examined. For both strains, identical restriction maps of kl and k2 DNA were obtained. Several restriction s

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The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribo