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TheHIS4 gene from the yeastKluyveromyces lactis

✍ Scribed by Freire-Picos, M. Angeles; Hampsey, Michael; Cerdán, M. Esperanza

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
147 KB
Volume
14
Category
Article
ISSN
0749-503X

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✦ Synopsis

The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb. The EMBL accession number of this gene is Y09503.

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