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Cloning and Characterization of theKlDIM1 Gene fromKluyveromyces lactis Encoding the m26A Dimethylase of the 18S rRNA

✍ Scribed by HOUSEN, ISABELLE; DEMONTÉ, DOMINIQUE; LAFONTAINE, DENIS; VANDENHAUTE, JEAN

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
154 KB
Volume
13
Category
Article
ISSN
0749-503X

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✦ Synopsis

The KlDIM1 gene encoding the m 2 6 A rRNA dimethylase was cloned from a Kluyveromyces lactis genomic library using a PCR amplicon from the Saccharomyces cerevisiae ScDIM1 gene as probe. The KlDIM1 gene encodes a 320-amino acid protein which shows 81% identity to ScDim1p from S. cerevisiae and 25% identity to ksgAp from Escherichia coli. Complementation of the kasugamycin-resistant ksgA-mutant of E. coli lacking dimethylase activity demonstrates that KlDim1p is the functional homologue of the bacterial enzyme. Multiple alignment of dimethylases from prokaryotes and yeasts shows that the two yeast enzymes display distinctive structural motives including a putative nuclear localization signal.

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