Karyological patterns and expression of malignancy in some homologous mouse somatic hybrid cells

✍ Scribed by Jean Belehradek Jr.; Georges Barski

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: French
• Weight: 573 KB
• Volume: 8
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910080102

✦ Synopsis

Abstract

A homologous mouse somatic hybrid cell line (HyEN) was developed in mixed cultures of C3H mouse malignant cell line crossed with a BALB/c mouse non‐tumorigenic cell line. An attempt was made to establish a possible relationship between the karyological features and die malignancy expression in this hybrid cell line as well as in six clones derived from it. As a rule, the karyotypes of the HyEN hybrid line and of its clones showed a marked dispersion of chromosome numbers and their modal values ranged from 80 to 129, the presumed “ideal” sum of the parental cell modes being 122. The HyEN line had a mode of 116 chromosomes and produced tumors when inoculated into histocompatible F~1~mice. Among the six randomly isolated hybrid clones studied, the only non‐tumor‐producing one had a mode of 112–118, quite similar to those of two other malignant clones. Three more clones were malignant and had modes of 80, 98 and 129 chromosomes. Early cultures of cells recovered from these different tumors obtained were likewise submitted to karyological analysis which showed a tendency to a decrease in chromosomal mode values after passage in vivo. A similar though less important tendency was noted following in vitro passages of the original HyEN line as well as of its clones. However, in spite of the general tendency toward chromosomal loss, the hybrid cells participating in the formation of tumors represented a wide range of chromosomal variants and no conclusion could be reached concerning a definite relationship between chromosomal deletion and malignancy of the hybrid cells.