Expression of nervous system antigen-3 by lines of mouse fibroblasts and kidney cells and continued expression in hybrid cells

✍ Scribed by Jane M. Doyle; Melitta Schachner; Richard L. Davidson

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 640 KB
• Volume: 93
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040930205

✦ Synopsis

Abstract

In a survey of the expression on cultured mouse cells of the cell surface antigen known as nervous system antigen‐3 (NS‐3), it was found that RAG, a renal adenocarcinoma line, expressed that antigen. It was also observed that 3T3, a fibroblast line of unknown tissue origin, expressed NS‐3. Cells of these two lines were hybridized with cells of two mouse L cell lines that did not express NS‐3. Four hybrid clones were tested for both the 3T3 × L cell cross and the RAG × L cell cross, and all the hybrids were found to be NS‐3 positive. All the hybrids had at least 40% as much activity as the NS‐3 positive parent. Of the four parental mouse cell lines used, only 3T3 expressed Thy‐1.2 antigen on the cell surface. In contrast to the continued expression of NS‐3 on hybrid cells, Thy‐1.2 antigen was not detectable on two clones of 3T3 × L cell hybrids that were tested.