Isolation of proline and hydroxyproline from fossil bone
β Scribed by Thomas W. Stafford Jr.; Raymond C. Duhamel; C. Vance Haynes Jr.; Klaus Brendel
- Book ID
- 118941327
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 409 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0024-3205
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π SIMILAR VOLUMES
The presence of hydroxyproline, and the determination of the ratio of the secondary amino acids proline to hydroxyproline, in amino acid hydrolysates specifically identifies collagen and collagen peptides. o-Phthalaldehyde, and then 9-fluorenylmethyl chloroformate, were used to carry out sequential
## Abstract Conditions are described for the treatment of proline with hydrogen peroxide, ascorbic acid, and ferrous ions to produce 3βhydroxyproline in 5β6% yield. Several reaction products are formed, but they can be separated chromatographically to yield pure transβ3βhydroxyproline. Synthesis of
## INTRODUCTIOX A recent report describes the presence of new isomers of hydroxyproline in collagenl. This finding poses several problems about the usefulness and the specificity of the methods commonly used for the determination of proline and hydroxyproline in normal or pathological tissues. The