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Separation of prolines and hydroxyprolines by gas chromatography

โœ Scribed by E. Mussini; F. Marcucci

Publisher
Elsevier Science
Year
1965
Tongue
English
Weight
290 KB
Volume
20
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

INTRODUCTIOX

A recent report describes the presence of new isomers of hydroxyproline in collagenl. This finding poses several problems about the usefulness and the specificity of the methods commonly used for the determination of proline and hydroxyproline in normal or pathological tissues. The methods available are usually based upon calorimetric determination+6.

It was therefore interesting, in relation to previous worl@, to develop a more specific procedure by means of gas chromatography for the separation of proline and hydroxyproline congeners.

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Rapid robust separation of hydroxyprolin
Rapid robust separation of hydroxyproline and proline
โœ Gene R. Nathans; Dennis R. Gere ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 650 KB

The presence of hydroxyproline, and the determination of the ratio of the secondary amino acids proline to hydroxyproline, in amino acid hydrolysates specifically identifies collagen and collagen peptides. o-Phthalaldehyde, and then 9-fluorenylmethyl chloroformate, were used to carry out sequential