๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Isolation of plasmid DNA from cell lysates by aqueous two-phase systems

โœ Scribed by S. C. Ribeiro; G. A. Monteiro; J. M. S. Cabral; D. M. F. Prazeres

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
175 KB
Volume
78
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Separation of genomic DNA, RNA, and open
Separation of genomic DNA, RNA, and open circular plasmid DNA from supercoiled plasmid DNA by combining denaturation, selective renaturation and aqueous two-phase extraction
โœ Andreas Frerix; Petra Geilenkirchen; Markus Mรผller; Maria-Regina Kula; Jรผrgen Hu ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 377 KB

## Abstract In the current study we developed a process for the capture of pDNA exploiting the ability of aqueous twoโ€phase systems to differentiate between different forms of DNA. In these systems scpDNA exhibits a near quantitative partitioning in the saltโ€rich bottom phase. The successive recove

Affinity-tagged green fluorescent protei
Affinity-tagged green fluorescent protein (GFP) extraction from a clarified E. coli cell lysate using a two-phase aqueous micellar system
โœ Priscila G. Mazzola; Henry Lam; Mojgan Kavoosi; Charles A. Haynes; Adalberto Pes ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 161 KB ๐Ÿ‘ 1 views

## Abstract Green fluorescent protein (GFP) has been proposed as an ideal choice for a proteinโ€based biological indicator for use in the validation of decontamination or disinfection treatments. In this article, we present a potentially scalable and costโ€effective way to purify recombinant GFP, pro