The structures of two minor oligosaccharides isolated from human milk have been investigated. By using f.a.b.-mass spectrometry, methylation analysis, and partial formolysis as the principal methods of structural investigation, these oligosaccharides were identified as IV2-alpha-Fuc-III6-alpha-NeuAc
Isolation of monosialylated oligosaccharides from human milk and structural analysis of three new compounds
✍ Scribed by Gunnar Grönberg; Peter Lipniunas; Torgny Lundgren; Karin Erlansson; Frank Lindh; Bo Nilsson
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 927 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The monosialyated oligosaccharide fraction from combined samples of human milk was fractionated by gel filtration, ion-exchange chromatography, and h.p.l.c. with triethylamine as an ion-pairing reagent. Among the twelve oligosaccharides isolated, three were new compounds for which the following structures were established on the basis of chemical analyses, f.a.b.-m.s., and n.m.r. spectroscopy.
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