Carbon nuclear magnetic resonance spectra of oligosaccharides isolated from human milk and ovarian cyst mucin
✍ Scribed by C.Allen Bush; Maximo M. Panitch; Virendra K. Dua; Thomas E. Rohr
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 801 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Natural abundance Carbon-13 nuclear magnetic resonance spectra at 20 MHz were reported for the three common human milk oligosaccharides, lacto-N-tetraose and lacto-N-fucopentaoses I and II, as well as for two related tetra-and hexasaccharide alditols isolated from the alkaline borohydride degradation products of an ovarian cyst glycoprotein. Spectral assignments made with the help of deuterium-induced shift (DIS), attached proton test (APT), and T, data indicated some very irregular glycosylation shifts which were attributed to effects of steric crowding and non-nearest-neighbor interactions. Samples as small as 10 pmol of oligosaccharide gave acceptable 20-MHz spectra with the use of a 5-mm probe coil. o 198s academic press, IOC.