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Isolation of DNA-free RNA, DNA, and proteins by cesium trifluoroacetate centrifugation

โœ Scribed by Hong Zhang; Huang-Tsu Chen; Vladimir Glisin

Book ID
116287305
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
218 KB
Volume
312
Category
Article
ISSN
0006-291X

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๐Ÿ“œ SIMILAR VOLUMES

Simultaneous banding of rat embryo DNA,
Simultaneous banding of rat embryo DNA, RNA, and protein in cesium trifluoroacetate gradients
โœ Philip E. Mirkes ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 761 KB

A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoracetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein. Under the conditions used DNA bande