Equilibrium centrifugation of DNA, RNA and poliovirus in density gradients of cesium oxalate and cesium acetate
โ Scribed by Laurence Zolotor; Reto Engler
- Book ID
- 115742822
- Publisher
- Elsevier Science
- Year
- 1967
- Weight
- 442 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0005-2787
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๐ SIMILAR VOLUMES
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoracetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein. Under the conditions used DNA bande
An efficient method for the isolation of RNA from cartilage is described. The difficulties in obtaining RNA from cartilage, a tissue of low cell density and high proteoglycan content, were overcome by making several modifications to the guanidine thiocyanate/cesium chloride method of RNA extraction.