The purification of TEM-ฮฒ-lactamase from
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R. R. Beitle; M. M. Ataai
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Article
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1991
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Springer-Verlag
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English
โ 234 KB
TEM-B-lactamase was purified by immobilized metal affinity chromatography from E. coil cell extracts. It was hypothesized that this protein could be purified in one step due to the abundence of metal binding residues. A pH gradient eluted the cell extract proteins. EDTA treatment released TEM-Blacta