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Isolation of compounds with affinity for copper from seawater using immobilized copper ion affinity chromatography

โœ Scribed by Andrew S. Gordon


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
707 KB
Volume
38
Category
Article
ISSN
0304-4203

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The purification of TEM-ฮฒ-lactamase from
โœ R. R. Beitle; M. M. Ataai ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 234 KB

TEM-B-lactamase was purified by immobilized metal affinity chromatography from E. coil cell extracts. It was hypothesized that this protein could be purified in one step due to the abundence of metal binding residues. A pH gradient eluted the cell extract proteins. EDTA treatment released TEM-Blacta