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Isolation of cold-sensitive Chinese hamster cells

✍ Scribed by Farber, Rosann A. ;Unrau, Paul

Publisher
Springer
Year
1975
Tongue
English
Weight
619 KB
Volume
138
Category
Article
ISSN
0026-8925

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✦ Synopsis

Six cold-sensitive variants have been isolated from Chinese hamster ovary cells by the BUdR-visible light selection technique. The properties of one of these lines have been studied in detail. This line stops dividing immediately after a shift from 39 degrees C to 33 degrees C though its doubling time at 39 degrees C is only slightly longer than that of wild-type cells. The rates of DNA and protein synthesis are severely reduced at 33 degrees C, but the rate of RNA synthesis is not significantly different from wild-type cells. This line may be defective in protein synthesis, but the results of sedimentation analysis indicate that it probably has normal ribosomal subunit assembly.

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