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Isolation of bacteria producing siderophores under alkaline conditions

✍ Scribed by D. J. Gascoyne; J. A. Connor; A. T. Bull


Publisher
Springer
Year
1991
Tongue
English
Weight
570 KB
Volume
36
Category
Article
ISSN
1432-0614

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✦ Synopsis


The isolation of bacteria producing siderophores under alkaline conditions is reported. Enrichment cultures initiated with samples from a number of alkaline environmental sources yielded 80 isolates. From this group selections were made on the basis of growth at high pH and the gallium-binding capacity of the siderophores. It was found that some isolates grew well and high concentrations of siderophore were detected whereas others grew well in the presence of much lower concentrations of siderophore. The effect of iron, gallium and aluminium on growth and siderophore production in batch culture was investigated for six isolates. The presence of iron greatly decreased the siderophore concentration in these cultures, whereas the response to added gallium or aluminium was dependent upon the isolate.


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