A gram-positive, nonfermentative, rod-shaped bacterium designated ST-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Neasurements of surface tension, critical micelle
Isolation and selection of biosurfactant-producing bacteria
โ Scribed by P. G. Carrillo; C. Mardaraz; S. I. Pitta-Alvarez; A. M. Giulietti
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 280 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-0972
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โฆ Synopsis
Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant-producing bacteria. Only 37 of 492 strains of different origins had haemolytic activity. These 37 strains, together with 49 non-haemolytic ones chosen at random, were studied for surface activity. Only five strains, all of them haemolytic, tested positive. Haemolysis and biosurfactant-production are thus probably associated.
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The isolation of bacteria producing siderophores under alkaline conditions is reported. Enrichment cultures initiated with samples from a number of alkaline environmental sources yielded 80 isolates. From this group selections were made on the basis of growth at high pH and the gallium-binding capac