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Isolation of biosurfactant-producing bacteria, product characterization, and evaluation

✍ Scribed by A. S. Abu-Ruwaida; I. M. Banat; S. Haditirto; A. Salem; M. Kadri


Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
533 KB
Volume
11
Category
Article
ISSN
0138-4988

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✦ Synopsis


A gram-positive, nonfermentative, rod-shaped bacterium designated ST-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Neasurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly giycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during exposure to high salinity (10% NaCI), elevated temperatures (120Β°C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86% of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.


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