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Isolation and characterization of lactic acid bacteria from lakes

✍ Scribed by Fujitoshi Yanagida; Yi-Sheng Chen; Masatoshi Yasaki

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
193 KB
Volume
47
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Lactic acid bacteria (LAB) were isolated from lake‐water samples collected at 7 lakes distributed in Yamanashi prefecture, Japan. Sampling was performed year round. 112 cultures were isolated and divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Phenotypic and biochemical characteristics identified eleven different bacterial groups (A to K), and the results showed that the isolates represented seven genera: Lactococcus, Leuconostoc, Enterococcus, Lactobacillus, Carnobacterium, Streptococcus, and Weissella. Lactococcus lactis subsp. lactis was the most abundant lactic acid bacteria found in these lakes. Furthermore, Lactococcus lactis subsp. lactis was also the most abundant lactic acid bacteria found throughout the year. Seasonal differences, numbers of isolates and the species of lactic acid bacteria were also recorded in this study. (Β© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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