Antimutagenicity of Lactic Acid Bacteria from El-Klila Cheese

✍ Scribed by Boubekri, Karima; Ohta, Yoshiyuki

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 539 KB
• Volume: 72
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199612)72:4<397::aid-jsfa673>3.0.co;2-e

✦ Synopsis

The successful research of lactic acid bacteria (LAB) reflect their potential application as anticarcinogens/antimutagens. We therefore studied in uitro binding assay of mutagenic heterocyclic aromatic amines (HCA) to 11 strains of LAB isolated from the Algerian traditional cheese, El-Klila. The mutagens were added at a concentration of 100 pg ml-' to methanolic suspensions of each lyophilised strains (5 mg), and incubated at 37°C for 15 min. HCA retained in samples were quantified by HPLC. The average binding (%) of 3-amino-1,4dimethyl-5H-pyrido-[4,3-b]-indole (Trp-P-1), 3-amino-l-methyl-5H-pyrido-[4,3b]-indole (Trp-P-2) and 3-amino-3-methyl-imidazo-[4,5-fl-quinoline (IQ) were 98.14 f 0.1, 96.45 k 0.13 and 34.90 k 0.5, respectively, and differed significantly ( P < 0.05). Pediococcus sp BK3-11 had the highest binding (%) activity to Trp-P-2 (99.58 f 0.25) and IQ (69.5 1.6). Lyophilised cytoplasm and cytoplasmic membrane of Pediococcus sp BK3-11 showed no binding activity, however the cell wall skeletons manifested significant binding (%) to Trp-P-2 (98.05 & 0.21) and IQ (59.56 f 0.7). The binding system was pH dependent and influenced by metal ions. The studies point out the antimutagenic cell components of lactic acid bacteria.