𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation of Agrobacterium Ti-plasmid regulatory mutants

✍ Scribed by Petit, Annik ;Temp�, Jacques


Publisher
Springer
Year
1978
Tongue
English
Weight
844 KB
Volume
167
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Genetic complementation of Agrobacterium
✍ Lundquist, Ronald C. ;Close, Timothy J. ;Kado, Clarence I. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 798 KB

Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant

Isolation of a recombination deficient A
✍ Klapwijk, P. M. ;van Beelen, P. ;Schilperoort, R. A. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 423 KB

The isolation of a recombination deficient (Rec-) strain of Agrobacterium tumefaciens is described. Strain LBA 4011 was mutagenized with nitrosoguanidine and after segregation 18,000 colonies were replica plated and UV irradiated. Twentytwo UV sensitive strains were isolated and tested for methylmet

In vivo transfer of the Ti-plasmid of Ag
✍ Holsters, M. ;Silva, B. ;Van Vliet, F. ;Hernalsteens, J. P. ;Genetello, C. ;Van 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 357 KB

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transf

Mutagenesis of plasmid DNA with hydroxyl
✍ Humphreys, G. O. ;Willshaw, Geraldine A. ;Smith, H. R. ;Anderson, E. S. 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 727 KB

An investigation of in vitro mutagenesis of plasmid DNA with hydroxylamine is described. The treated plasmid DNA was used to transform Escherichia coli K12. Mutants of the plasmid NTP3, which codes for resistance to ampicillin and sulphonamides, were isolated and characterised. They were classified