Isolation of Agrobacterium Ti-plasmid regulatory mutants
✍ Scribed by Petit, Annik ;Temp�, Jacques
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 844 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0026-8925
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The isolation of a recombination deficient (Rec-) strain of Agrobacterium tumefaciens is described. Strain LBA 4011 was mutagenized with nitrosoguanidine and after segregation 18,000 colonies were replica plated and UV irradiated. Twentytwo UV sensitive strains were isolated and tested for methylmet
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