✦ LIBER ✦

Isolation of a poplar and anArabidopsis thalianadihydrodipicolinate synthase cDNA clone

✍ Scribed by Marc Vauterin; Michel Jacobs

Publisher: Springer
Year: 1994
Tongue: English
Weight: 835 KB
Volume: 25
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00043882

No coin nor oath required. For personal study only.

✦ Synopsis

A poplar D H D P S cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the poplar and maize D H D P S cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Arabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis D H D P S genomic and cDNA clone.

📜 SIMILAR VOLUMES

Isolation and nucleotide sequence of a c

Isolation and nucleotide sequence of a cDNA clone encoding the beta subunit of mitochondrial ATP synthase fromHevea brasiliensis

✍ Mee -Len Chye; Chio -Tee Tan 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 182 KB

Isolation and characterization of a wate

Isolation and characterization of a water-stress-inducible cDNA clone fromSolanum chacoense

✍ Dániel Silhavy; György Hutvágner; Endre Barta; Zsófia Bánfalvi 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 783 KB

A rich source of valuable genes are wild species. Solanum chacoense Bitter with its extreme resistance to viruses, insects and drought, is a good example. In the present study, a stress gene, designated DS2, has been isolated from S. chacoense. We have shown that the expression of the gene is organ

Isolation and characterization of a rice

Isolation and characterization of a rice cDNA clone encoding ATP/ADP translocator

✍ Hiroshi Hashimoto; Ritsuko Nishi; Masaaki Umeda; Hirofumi Uchimiya; Atsushi Kato 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 202 KB

We isolated a rice cDNA clone which encodes an open reading frame of 382 amino acids. Its deduced amino acid sequence corresponds to an ATP/ADP translocator protein. Its homology with a maize ATP/ADP translocator was 83.9~/o in nucleotide sequence, and 90.2~o of the amino acid level. Expression of t

Isolation and characterization of a toba

Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase

✍ Cathal Wilson; Norbert Eller; Anton Gartner; Oscar Vicente; Erwin Heberle-Bors 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 809 KB

We have isolated and sequenced a MAP (mitogen-activated protein) kinase-type cDNA from a tobacco (Nicotiana tabacum L.) cell suspension cDNA library by screening with a PCR fragment amplified from the same library with oligonucleotide primers corresponding to two sequences conserved in yeast and ani

Isolation and characterization of a cDNA

Isolation and characterization of a cDNA clone encoding the anti-viral protein fromPhytolacca americana

✍ Q. Lin; Z. C. Chen; J. F. Antoniw; R. F. White 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 421 KB

Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-c

Isolation and molecular characterisation

Isolation and molecular characterisation of a tuberisation-related cDNA clone from potato (Solanum tuberosumL.)

✍ M. A. Taylor; A. Kumar; L. A. George; H. V. Davies 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 775 KB

A cDNA clone of a gene which shows a large increase in transcript level in the stolon tip during the early stages of tuberisation in potato (Solanum tuberosum) has been isolated by differential screening. This gene is also expressed at low levels in other parts of the plant including leaves and stem