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Isolation of a lactic dehydrogenase-A-deficient CHO-K1 mutant by nylon cloth replica plating

โœ Scribed by T. D. Stamato; Carol Jones


Book ID
112558883
Publisher
Springer
Year
1977
Tongue
English
Weight
844 KB
Volume
3
Category
Article
ISSN
1572-9931

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Isolation of mammalian cell mutants defi
โœ Dieter Wild; Bernard Hellkuhl ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer ๐ŸŒ English โš– 841 KB

A replica-plating technique is described which was used for the isolation of G-6-PD-deficient mutants in cultures of mutagen-treated Chinese hamster cells. Mutants were recognized by their failure to stain in a histochemical G-6-PD-specific staining reaction. Four mutants were isolated and character