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Isolation of mammalian cell mutants deficient in glucose 6-phosphate dehydrogenase by means of a replica-plating technique

✍ Scribed by Dieter Wild; Bernard Hellkuhl

Book ID
104709898
Publisher
Springer
Year
1976
Tongue
English
Weight
841 KB
Volume
32
Category
Article
ISSN
0340-6717

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✦ Synopsis

A replica-plating technique is described which was used for the isolation of G-6-PD-deficient mutants in cultures of mutagen-treated Chinese hamster cells. Mutants were recognized by their failure to stain in a histochemical G-6-PD-specific staining reaction. Four mutants were isolated and characterized by growth properties, stability of their variant phenotypes, and reduced G-6-PD activity. One of these mutants on electrophoresis exhibited a variant G-6-PD and thus is very likely the result of a mutation in the structural gene for G-6-PD.

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