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Isolation of a spontaneous CHO amino acid transport mutant by a combination of tritium suicide and replica plating

✍ Scribed by Anne H. Dantzig; Carolyn W. Slayman; Edward A. Adelberg


Book ID
112558761
Publisher
Springer
Year
1982
Tongue
English
Weight
615 KB
Volume
8
Category
Article
ISSN
1572-9931

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A cDNA clone named L21 was isolated from L6 rat muscle cells by complementation of a yeast proline transport mutant. L21 cDNA has 2,268 bp and codes for a peptide of 228 amino acids with four potential transmembrane domains. The amino acid sequence of L21 shows no homology to any known proteins. Exp